Skip to main content
. 2016 Aug 8;11(8):e0160783. doi: 10.1371/journal.pone.0160783

Fig 4. TL induces oxidative stress in p53-Deficient HCT116 cells.

Fig 4

(A,B,C) TL treatment led to nuclear accumulation of NRF2 and its target genes (HO1 and NQO1). (A) HCT116 p53+/+ and p53-/- cells were treated with TL (50nM for 30 and 60 minutes). Nuclear extracts were prepared and subjected to immunoblotting with NRF2, NQO1 and HO1. Representative Immunoblots are presented. (B,C,D) Relative protein quantity normalized to PCNA is presented (**p<0.01, n = 3). (E) TL decreases glutathione content. The glutathione levels were measured in cell lysates from TL-induced and control HCT116p53+/+ and p53-/- cells. The levels were normalized with protein content and the data was represented as relative content of %control (*p<0.05). (F-H) TL inhibits SOD2 levels and activity in p53-depleted cells. (F,G) Immunoblotting for Mn-SOD (SOD2) was performed in cell lysates from HCT116 p53+/+ and p53-/- stimulated with DMSO or TL. Relative SOD2 protein levels normalized to beta-actin. (H) SOD activity was measured in cell lysates from F. The data was presented as %activity (mean±SD; *p<0.05; n = 3).