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. 2016 Aug 8;11(8):e0160783. doi: 10.1371/journal.pone.0160783

Fig 6. TL induces hyperacetylation of ETC complexes through mitochondrial Sirt3.

Fig 6

(A,B) P53 containing and deficient HCT116 cells were treated with TL (50 nM for 6 hours). Immunoprecipitates of anti-acetylayed lysine were immunoblotted with hNDUFA9 (A, top panel) or succinate dehydrogenase (hSDHA) (B, top panel). Whole-cell lysates were immunoblotted with hNDUFA9 (A, lower panel) and SDHA (B, lower Panel) for the input. (C) TL down-regulates SIRT3 expression. Whole-cell lysates from HCT116 cells (p53 +/+ and p53-/-) induced by DMSO or TL were immunoblotted with anti-SIRT3, P53, and beta-actin (top panel). Relative quantity of SIRT3 was computed after normalization with beta-actin (mean±SD; *p<0.05; n = 3).