Fig 1. Microarray analysis of HIV-1 infected patient samples.

The microarray chip was probed with unvaccinated (non-vaccine arm) and vaccinated (vaccine arm) patient plasma samples (week 0, week 36, and week 56) at a dilution factor of 1:100. HIV-1 specific gp140 envelope proteins were printed at a concentration of 0.01 mg/mL, which corresponds to 0.01 ng per spot. HIVIG and IVIG were included as a positive control and negative control, respectively. Antibody signal intensities were color-coded using green (>0.05–0.5 x 10³) for weak, yellow (>0.5–1.5 x 10⁴) for intermediate, orange (>1.5–3.0 x 10⁴) and red (>3.0 x 10⁴) for strong interaction. Non-specific binding was indicated as white (<0.5 x 10²) boxes. Raw data is indicated in S3 Fig.