Fig 5. Mutation K126R of TTR alters the SUMOylation patterns of TTR_Ubc9^mut.

Non-mutated TTR (T) or K126R mutant of TTR (T¹²⁶), fused to either the K14,153,154R mutant of Ubc9 (U^mut) at the N-terminus (T_U^mut and T¹²⁶_U^mut) (A) or the C-terminus (U^mut_T and U^mut_T¹²⁶) (B) was co-expressed in HEK293 cells with EGFP-labelled SUMO-1 (ES1) or SUMO-3 (ES3). The cell lysates were analysed by WB using anti-TTR antibodies (αTTR). Arrows with letters (a, b, c, d, or e) indicate band differences caused by the K126R mutation of TTR.