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. 2016 Aug 8;11(8):e0160536. doi: 10.1371/journal.pone.0160536

Fig 8. Non-fused and Ubc9-fused TTR influences general SUMOylation.

Fig 8

Each of protein—non-fused TTR (TTR), TTR fused to the N-terminus of Ubc9 (TTR_Ubc9), and non-fused Ubc9 or EGFP (control)—was co-expressed with EGFP-labelled SUMO-1 (ES1) or SUMO-3 (ES3) in HEK293 cells. The cell lysates were analysed by WB using anti-GFP antibodies. The histograms present the intensities of the bands measured in the lanes containing the lysates of the cells expressing the indicated proteins. The intensity of each band is plotted against its distance from the top of the separating gel (marked with 0). The superimposed histograms represent the samples run on the same gel (A, B and E) or on parallel gels (C and D). The arrows represent the position of the 95-kDa protein marker. The asterisks indicate free ES1 and ES3; the double asterisks show the Ubc9 bands. N marks the region containing unspecific bands.