Table 6.
Levels of oxidatively induced DNA bases in Ku70–/– genomic DNA 24 hr after damage inductiona.
| Exposure | DNA lesion/106 DNA bases (mean ± SD, n > 3)b | |||
|---|---|---|---|---|
| ThyGly | FapyAde | FapyGua | 8-oxoGua | |
| Control | 5.32 ± 0.80 | 3.61 ± 0.26 | 3.81 ± 0.83 | 3.25 ± 0.01 |
| BPA | 2.54 ± 0.69 | 2.75 ± 1.74 | 3.71 ± 1.88 | 3.09 ± 0.57 |
| KBrO3 | 3.54 ± 0.27 | 3.21± 0.32 | 3.77 ± 1.31 | 2.84 ± 0.55 |
| BPA + KBrO3 | 5.38 ± 1.74 | 3.35 ± 0.31 | 6.00 ± 2.02 | 2.80 ± 0.80 |
| aSamples treated with 150 μM BPA only were exposed for 25 hr; those treated with 20 mM KBrO3 only were exposed for 1 hr, then medium was replaced for 23 hr; and co-exposed samples were exposed to 150 μM BPA for 1 hr, then exposed to 150 μM BPA + 20 mM of KBrO3 for 1 hr, followed by exposure for 23 hr to 150 μM BPA only. bMeasurements were made by GC/MS as described in “Methods.” | ||||