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. 2016 Jan 13;124(8):1214–1226. doi: 10.1289/ehp.1510352

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ToxPi chemicals induce adipogenesis in 3T3-L1 preadipocytes. Adipogenesis was induced in 3T3-L1 cells according to Figure S2. 3T3-L1 cells were exposed to adipogenic cocktail for 2 days, then exposed to the test chemicals for 5 days. Differentiated cells were fixed and stained with Nile red and Hoechst 33342. Lipid accumulation was quantified in cells by measuring Nile red fluorescence normalized by cell number (Hoechst). Rosiglitazone and tributyltin serve as positive control adipogenic chemicals. One-way ANOVA was conducted for ToxPi chemical treatment groups and DMSO vehicle, followed by Dunnett’s post hoc test: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to vehicle. Unpaired t-test was conducted for the positive controls rosiglitazone, tributyltin versus vehicle: ^### p ≤ 0.001.