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. 2016 Jan 13;124(8):1214–1226. doi: 10.1289/ehp.1510352

Figure 7.

Figure 7

ToxPi chemicals quinoxyfen and fludioxonil induce adipogenesis in mBMSCs. Adipogenesis was induced in mouse bone marrow derived mesenchymal stem cells (mBMSCs) according to Figure S2. mBMSCs were exposed to adipogenic cocktail plus test chemicals or positive controls for 14 days. Differentiated cells were fixed and stained with Nile red and Hoechst 33342. Lipid accumulation was quantified in differentiated cells by measuring Nile red fluorescence normalized by cell number (Hoechst). Rosiglitazone (ROSI) and tributyltin (TBT) serve as positive control adipogenic chemicals. mBMSCs were homogenized in TriPure, total RNA was isolated, reverse transcribed, and QPCR was performed. Gene expression was determined by the 2– ΔΔ CT method using 36b4 as the reference gene. Data are reported as fold induction over 0.1% DMSO vehicle controls ± S.E.M using standard propagation of error. Primer sequences can be found in Table S4. One-way ANOVA was conducted for ToxPi chemical treatment groups and DMSO vehicle, followed by Dunnett’s post hoc test: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to vehicle. Unpaired t-test was conducted for the positive controls rosiglitazone, tributyltin versus vehicle: # p ≤ 0.05, ## p ≤ 0.01, ### p ≤ 0.001.