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. Author manuscript; available in PMC: 2016 Aug 8.
Published in final edited form as: Cell Microbiol. 2011 Sep 14;13(12):1881–1893. doi: 10.1111/j.1462-5822.2011.01666.x

Fig. 3.

Fig. 3

EspT induces Il-8 and Il-1β secretion via JNK and Erk activation. U937 cells were pre-treated with SB203580 (SB), PD98059 (PD) or SP600125 (SP) 1 h before infection. PD and SP significantly reduce the level of EspT-mediated Il-8 (A) and Il-1β (B) secretion. Western blot of whole-cell lysates probed with antibodies directed against phospho-Erk (pErk) (C) and phospho-JNK (pJNK) (D) and normalized using a-tubulin antibody (tubulin) show that EPEC expressing EspT triggers phosphorylation of both Erk and JNK MAPKs. Pre-treatment with Wortmannin (Wort) or LY294002 (LY) 1 h before infection shows no significant changes on the level of EspT-induced Il-8 secretion (E). Data are means + SD and are representative of at least three independent experiments; P value determined by Student’s T-test (*P < 0.05) comparing EspT strain with all other conditions as indicated.