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. Author manuscript; available in PMC: 2016 Aug 8.
Published in final edited form as: Cell Microbiol. 2011 Sep 14;13(12):1881–1893. doi: 10.1111/j.1462-5822.2011.01666.x

Fig. 5.

Fig. 5

EspT-mediated Il-8 production is regulated via RAC1.

A. Alignment of IpgB1, Map EspM2 and EspT effector proteins; the point mutations at position W63 and Q117 are indicated in red.

B. U937 cells were infected with E2348/69 or E2348/69 expressing EspTWT, EspTW/A or EspTQ/Y. EspTW/A and EspTQ/Y mutants drastically reduced the level of EspT-mediated Il-8 secretion.

C and D. U937 cells were transfected with non-targeting (NT) siRNA or siRNA targeted against Rac1 (SiRac1) or Cdc42 (SiCdc42) for 48 h. Successful knock-down was confirmed by Western blotting (C). Transfected U937 cells were either uninfected (U/I) or infected with E2348/69 expressing EspT (EspT). siRac1 inhibits the level of EspT-mediated Il-8 secretion while siCdc42 showed no effect on the level of Il-8 secretion.

E. Pre-treatment with NSC23766 (NSC) for 1 h reduced the level of EspT-mediated Il-8 secretion. Data are means + SD and are representative of at least three independent experiments; P value determined by Student’s T-test (*P < 0.05) comparing EspT strain with all other conditions as indicated.