Figure 9.

Effect of Sal, AgNPs, or both Sal and AgNPs on caspase-3 activity.

Notes: The cells were treated with Sal (3 µM), AgNPs (4 µg/mL), or both Sal (3 µM) and AgNPs (4 µg/mL) with or without the caspase-3 inhibitor Ac-DEVD-CHO for 24 hours. The concentration of p-nitroanilide released from the substrate was calculated from the absorbance at 405 nm. The results are expressed as mean ± standard deviation of three independent experiments. The treated groups showed statistically significant differences from the control group by the Student’s t-test (*P<0.05).

Abbreviations: AgNPs, silver nanoparticles; Con, control; Sal, salinomycin.