Figure 1.

Identification of Cardiomyocyte RBPs

(A) Schematic of mRNA interactome capture and RBDmap approaches. Proteins identified by one or both approaches constituted a superset of cardiomyocyte RBPs.

(B) RNA-protein complexes captured on oligo(dT) beads were digested with proteinase K and RNA recovery levels monitored by quantitative PCR. Shown are averages of six biological replicates. Error bars, SD.

(C and D) Complexes captured on oligo(dT) beads were eluted by RNase-digestion, resolved by SDS-PAGE alongside input WCL (percentage equivalent to loaded eluate amount as indicated), and analyzed by western blot (C; see Experimental Procedures for antibody details) or silver stain (D). Results are representative of three independent interactome capture experiments. (See Figures S1A and S1B for equivalent RBDmap controls.)

(E) Proportion of cardiomyocyte RBPs or WCL proteins with GO annotation “RNA binding” or RNA-related annotations (see Supplemental Experimental Procedures for details on annotation sources). Left, cardiomyocyte RBPs; right, WCL.

(F) Analyses as in (E) for RBDs.

(G) Matrix plot of enriched/depleted KEGG pathways among RBP groups as defined in (E), compared to WCL.

See also Figures S1 and S2 and Table S1.