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. 2016 Aug 9;3:40. doi: 10.3389/fmolb.2016.00040

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Copyright © 2016 Anzai, Toichi, Tokuda, Mukaiyama, Akiyama and Furukawa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Vitamin D derivatives as potent inhibitors for the formation of insoluble apo-SOD1(G37R)^S-S aggregates. (A–F) The turbidity increase of 20 μM apo-SOD1(G37R)^S-S in the presence of 1 M Gdn is shown in the presence of the following compounds (red filled circles): (A) alfacalcidol, (B) calcidol, (C) calcitriol, (D) calcipotriol, (E) calciferol, and (F) cholecalciferol. In each panel, the aggregation kinetics without any drugs was shown as gray filled circles. The kinetics was monitored by changes in the solution turbidity (the absorbance at 350 nm), and the final concentration of drugs added was 20 μg/mL. Experiments were repeated more than three times to estimate error bars (standard deviation). Structures of the chemical compounds were also shown below the panels.