Table 2.

Leaf total N content as affected by nitrogen supply.

Years	Scion/rootstock combination	Nitrate supply	Leaf nitrogen nitrogen Content (%)	Petiole nitrogen content (%)	Analysis of variance	Leaf nitrogen content (%)	Petiole nitrogen content (%)
2013	CS/RGM	N–	1.00 ± 0.10a	0.23 ± 0.01	Years (Y)	**	NS
		N+	1.77 ± 0.41b	0.37 ± 0.12	Treatment (T)	***	*
	CS/110R	N–	1.14 ± 0.17a	0.25 ± 0.05	Variety (V)	**	NS
		N+	1.79 ± 0.30b	0.48 ± 0.18	Rootstock (R)	NS	NS
	PN/RGM	N–	1.10±	0.28±	Y/T	NS	NS
		N+	1.76±	0.37±	Y/V	NS	NS
	PN/110R	N–	1.12±	0.21±	V/R	NS	NS
		N+	1.87±	0.37±	T/V	NS	NS
2014	CS/RGM	N–	1.10 ± 0.08a	0.38 ± 0.10	Y/R	NS	NS
		N+	2.10 ± 0.09b	0.66 ± 0.10	T/R	NS	NS
	CS/110R	N–	1.17 ± 0.20a	0.39 ± 0.04	Y/T/V	NS	NS
		N+	2.02 ± 0.07b	0.55 ± 0.12	Y/T/R	NS	NS
	PN/RGM	N–	1.43 ± 0.08a	0.33 ± 0.01	Y/V/R	NS	NS
		N+	2.22 ± 0.09b	0.63 ± 0.12	T/V/R	NS	NS
	PN/110R	N–	1.36 ± 0.04a	0.30 ± 0.02	Y/T/V/R	NS	NS
		N+	2.43 ± 0.02b	0.60 ± 0.08

^±Unique value. Values are means of 3 independent replicates + SE. N−: 0.8 mM N; N+: 3.6 mM N. One factor (N treatment) Anova tests were made, as well as Tukey tests. For each rootstock/variety combination, a and b indicate significantly different values between N− and N+ treatment. ^±Unique value. Statistical analyses were done using an analysis of variance with years (Y), rootstock (R), treatment (T), variety (V), and their interaction effects (ns, P > 0.05;

^*P < 0.05;

^**P < 0.01;

^***P < 0.001).