FIGURE 6.

Effect of bovine serum albumin (BSA) on the digestion of histone mixture by P. gingivalis proteases. Histone mixture (42 μg/ml) was digested by 4 μl of 1:10 diluted P. gingivalis supernatant in the presence or absence of 0–12 μM BSA. The effect of BSA on histone digestion was measured by calculating the undigested histone proportion in SDS-PAGE. Digestions were carried out at 20°C for 30 min, samples were run on SDS-PAGE and evaluated as described in section “Materials and Methods”. The presented data are mean and standard deviation of three independent experiments.