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. 2016 Aug 9;7:1156. doi: 10.3389/fpls.2016.01156

FIGURE 2.

FIGURE 2

Western blot of the expression of BHC, BLC, and Bevacizumab mAb in transgenic rice callus. The BLC, BHC, and Bevacizumab mAb were detected with a goat anti-human IgG (LC and HC) antibody under reducing (A,B) and non-reducing conditions (C). (A) BHC and BLC were expressed from different gene expression cassette, (B,C) BHC and BLC were expressed as a polyprotein separated with the 2A peptide. PC is a positive control with a commercial Bevacizumab mAb; NC is a negative control with the total soluble proteins from non-transformed callus; lanes 1–8 in (A), 1–5 and 6–9 in (B,C) are total soluble proteins from different transgenic events transformed with pUNBHLC, pUNBevaHL, and pUNBevaHL-KDEL, respectively. Protein molecular weights are indicated on the left side of each panel. BHC (50 kDa), BLC (25 kDa), and mAb (150 kDa) are indicated on the right side of each panel.