Table 2.
Mass spectrometric analysis of glycopeptides from purified mAb after tryptic digestion.
| Glycoform | Relative abundance (%) | |
|---|---|---|
| pUNBevaHL | pUNBevaHL-KDEL | |
| Non-glycosylated | 2.7 | 10.8 |
| Single GlcNAc (Gn) | 16.3 | 26.1 |
| Man3 (M3Gn2) | 9.4 | 1.6 |
| Man4 (M4Gn) | 1.9 | 1.2 |
| Man5 (M5Gn2) | 2.3 | 1.9 |
| Man6 (M6Gn2) | 1.7 | 1.6 |
| Man7 (M7Gn2 | 2.6 | 2.4 |
| Man8 (M8Gn2) | 4.0 | 5.7 |
| Man9 (M9Gn2) | 2.4 | 1.9 |
| MMXF (M3Gn2X1F1) | 19.4 | 14.6 |
| GnMXF (M3Gn3X1F1) | 7.6 | 5.1 |
| GnGnXF (M3Gn4X1F1) | 29.7 | 27.1 |
GlcNAc, N-acetylglucosamine (Gn); F, fucose; Man, mannose (M); X, xylose.