FIGURE 2.

Subcellular localization of TaPARG-2D in wheat protoplasts (A) and tobacco leaf cells (B). The vector control (35S::GFP) and fusion protein construct 35S::TaPARG-2D::GFP were introduced into wheat protoplasts and tobacco leaf cells, respectively. For wheat protoplast transformation, GFP was detected in transformed wheat lines after 24 h with a laser scanning confocal microscope; for tobacco, GFP was detected in leaf cells 4 days after transformation. Images are in dark field (1, 5, 9, and 12), bright field (2, 6, 10, and 13), and combined (4, 8, 11, and 14). Chloroplasts are indicated by red auto-fluorescence (3 and 7). Scale bars: 20 μm for wheat protoplasts; 50 μm for tobacco leaf cells.