Figure 2. Effects of zirconia with or without an intermediate cement layer on IL-6 expression by (a) L929 and (b) MC3T3-E1 cells. The graphs in (c) and (d) show the densitometric quantification of protein expression of the bands shown in (a) and (b). The results are presented as fold increases relative to control. The graphs in e and f present ELISA results.

^*Significant difference from the negative control group at each time period, according to Tukey tests. Different lower case letters indicate significant between group differences.

GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin-6; PCR, polymerase chain reaction; CTR, Group 1, negative control; ZR, Group 2, positive control; ZR + RU, Group 3, zirconia with RelyX U200; ZR + FU, Group 4, zirconia with FujiCEM 2; ZR + PF, Group 5, zirconia with Panavia F 2.0.