Table 2.
DADS concentration (mM) | Mitotic index (%) | Dividing cells in different phases of mitosis | ||||
---|---|---|---|---|---|---|
Prophase (%) | Metaphase (%) | Anaphase (%) | Telophase (%) | |||
24 h | 0 | 4.38 ± 0.24b | 3.04 ± 0.21a | 0.90 ± 0.06bc | 0.19 ± 0.04ab | 0.25 ± 0.06ab |
0.04 | 4.09 ± 0.21b | 2.61 ± 0.14b | 1.04 ± 0.09b | 0.21 ± 0.02a | 0.23 ± 0.04ab | |
0.21 | 5.01 ± 0.21a | 3.26 ± 0.13a | 1.33 ± 0.10a | 0.18 ± 0.04ab | 0.23 ± 0.07ab | |
0.41 | 4.62 ± 0.22ab | 3.15 ± 0.19a | 1.05 ± 0.08b | 0.12 ± 0.03bc | 0.30 ± 0.04a | |
1.45 | 3.24 ± 0.11c | 2.36 ± 0.10bc | 0.72 ± 0.06c | 0.08 ± 0.02c | 0.09 ± 0.02c | |
6.20 | 3.09 ± 0.18c | 2.08 ± 0.11cd | 0.71 ± 0.07c | 0.14 ± 0.02abc | 0.16 ± 0.03bc | |
12.40 | 2.34 ± 0.14d | 1.80 ± 0.09d | 0.39 ± 0.08d | 0.09 ± 0.02c | 0.07 ± 0.03c | |
48 h | 0 | 3.81 ± 0.24ab | 2.74 ± 0.29ab | 0.78 ± 0.06b | 0.11 ± 0.02b | 0.19 ± 0.05ab |
0.04 | 4.03 ± 0.25a | 2.71 ± 0.17ab | 1.04 ± 0.09a | 0.12 ± 0.03b | 0.16 ± 0.04abc | |
0.21 | 4.16 ± 0.22a | 2.85 ± 0.14a | 0.88 ± 0.07ab | 0.21 ± 0.04a | 0.23 ± 0.02ab | |
0.41 | 4.12 ± 0.21a | 2.66 ± 0.15ab | 1.07 ± 0.07a | 0.13 ± 0.02b | 0.26 ± 0.05a | |
1.45 | 3.33 ± 0.22bc | 2.31 ± 0.12bc | 0.77 ± 0.09b | 0.12 ± 0.03b | 0.13 ± 0.03bc | |
6.20 | 3.11 ± 0.16c | 2.15 ± 0.09cd | 0.70 ± 0.05bc | 0.13 ± 0.02b | 0.14 ± 0.03bc | |
12.40 | 2.35 ± 0.22d | 1.68 ± 0.18d | 0.50 ± 0.06c | 0.11 ± 0.02b | 0.06 ± 0.02c |
Mitotic index and frequencies of each mitotic phase were calculated for 10,000 cells. Values for particular treatment (in columns, mean ± SE) followed by different small letters are significantly different at the 0.05 level, N = 10 (LSD test). The bold values represent the important differences between treatments and the water control.