Table 3.
The survival rates of the mice which were immunized subcutaneously with tATA4 mixed with or without adjuvant 3 times at 3 wk intervals after challenge of different doses of abrin
| Survival rate (total:10)a |
||||
|---|---|---|---|---|
| Groups | 20 LD50 | 30 LD50 | 40 LD50 | 50 LD50 |
| tATA4+Imject® Alum | 100% | 100% | 80% | 70% |
| tATA4+SPO1 | 100% | 100% | 100% | 70% |
| tATA4 | 100% | 80% | 60% | 0 |
| Controlb | 0 | 0 | - | - |
a: group of 10 mice were challenged (i.p. injection) one week after the third immunization and survival status were recorded each day for ten days post-challenge and the survival rate were calculated at day 10; b: mice were immunized with PBS alone subcutaneously. In multivariable logistic regression, significant difference of survival rates has been noticed between “tATA4 with adjuvant” groups and “tATA4” group (P < 0.01), but not between two adjuvant groups (P = 0.406), which indicated that the two adjuvants might have equal effect in this study. “-”: data not done.