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HPLC-SEC chromatograms of pneumococcal-PD/TT/DT bulk conjugates and carrier proteins stability samples. Serotypes 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, 23F and PD, TT, DT (A to M) were stored at temperatures of -20, 4, 37, or 56°C for 5 wk, as labeled, or exposed to repeated freeze-thawing (F/T). Samples were loaded onto a TSK5000 PWXL column and eluted in PBS, pH7.4 with a flow rate 0.3 ml/min.
