Table 1.
Properties of differential spectra and Kd values of propiconazole against CYP90D1.
| Δλmax (nm) | Δλmin (nm) | Kd (μM) | |
|---|---|---|---|
| Experiment 1 | 437 | 414 | 0.75 |
| Experiment 2 | 436 | 415 | 0.77 |
Data obtained from the present work provided evidence for the first time on the biochemical elucidation of the target site of Pcz in BR biosynthesis. Our results clearly indicated that Pcz binds to CYP90D1. The feeding of BR biosynthesis intermediates provided evidence that Pcz targets side chain hydroxylation in BR biosynthesis.