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. 2016 Aug 9;6:31140. doi: 10.1038/srep31140

Figure 3. Astrocytes and HEK293 cells grown on P1M3DH.

Figure 3

(a) Epifluorescence microscopy of cerebellar astrocytes expressing mCherry. (b) Laser confocal image of astrocytes grown on P1M3DH. (c) Epifluorescence microscopy of HEK293 cells expressing eGFP grown on P1M3DH scaffolds. (d) Laser confocal image of HEK293 cells on P1M3DH. Notice that the autofluorescence emitted by the scaffold under the epifluorescence microscope is eliminated by proper filtering under the confocal microscope. Astrocytes imaged in B show complex morphologies, from two to multiple processes. Dark areas on B and D correspond to deep cavities on the surface of the scaffold, as those revealed by SEM.