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. 2016 Aug 9;6:31088. doi: 10.1038/srep31088

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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mRNA expression of SRY (a) and other (c) testis or ovarian determining factors were examined by QPCR. Significance of expression changes is indicated (one-way ANOVA). (b) Immunohistochemical analysis of SRY, VASA, SOX9, AMH and DMRT1 in E34 fetal gonads. The cells were counterstained with hematoxylin. Scale bars in = 150 μm; in inset = 40 μm.