Figure 5. SRY methylation and gene expression of sex-determining genes in 1 year postnatal fetal gonads of XY^wt, XX^wt, and XY^DSD cloned dogs.

(a) Promoter methylation status of SRY gene at 1 year postnatal gonad in the XY^wt and XY^DSD cloned dogs, using Bisulfite Sequencing PCR (BSP). (c) mRNA Expression of testis or ovarian determining factors were examined by QPCR. Significance of expression changes is indicated (one-way ANOVA). (b) Immunohistochemical analysis of SRY, SOX9, AMH, AMHRII and Inhibitinα. The cells were counterstained with hematoxylin. Scale bars in = 150 μm.