Figure 1.

PLL increases the number and intensity of green fluorescent cells in vitro. Primary murine osteoblasts were cultured and transduced with a GFP-encoding adenovirus at the indicated MOIs in the presence or absence of PLL. Cells were labeled with DiI (red) and fluorescence was detected by fluorescence microscopy. As negative controls, sets of cells were cultured with or without PLL but were not transduced with adenovirus. Data are from a representative experiment. Scale bar=20 μm. GFP, green fluorescence protein; MOI, multiplicities of infection; PLL, poly-l-lysine.