Figure 2.

PLL markedly enhances adenoviral transduction of osteoblasts and BMSCs in vitro. (a) Flow cytometry was performed on primary murine osteoblasts and BMSCs that had been transduced with a GFP-encoding adenovirus at the indicated MOIs in the presence or absence of PLL. The percentage of GFP+ cells is indicated for each condition. Quantitation of the percentage of GFP-positive cells for osteoblasts (OBs) and BMSCs at each MOI is shown in the histogram. (b) The median fluorescent intensity of the GFP+ population of long bone osteoblasts (LOBs) and BMSCs is shown. Data are from a representative experiment. BMSC, bone marrow stromal cell; GFP, green fluorescence protein; MOI, multiplicities of infection; PLL, poly-l-lysine.