Figure 3.

Adenoviral transduction with PLL does not affect cell viability or osteoblast differentiation. (a) The viability of primary osteoblasts was assessed with a colorimetric CCK-8 cell viability assay at the indicated MOIs in the presence or absence of PLL. Cells were transduced with GFP (negative control) or Cre recombinase expressing adenovirus as indicated. Viability was assessed 72 h post transduction. Pathlength corrected OD450 values are shown as mean±s.d. No statistical significance between samples was observed. (b) mRNA levels of osteoblast genes were determined by quantitative RT-PCR from cells transduced with Ad-GFP (MOI=5) at the presence or absence of PLL and then cultured in osteogenic media for 7 days. No statistical significance between samples was observed. CCK-8, Cell Counting Kit-8; GFP, green fluorescence protein; MOI, multiplicities of infection; PLL, poly-l-lysine; RT-PCR, reverse transcription–PCR.