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. 2016 Aug 9;6:31281. doi: 10.1038/srep31281

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Copyright © 2016, The Author(s)

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(a,b) Sperm vitality was measured by the hypo-osmotic swelling test following incubation with PCB 153 (a) and DEHP (b) [n = 7 dogs]. (c) Sperm DNA fragmentation was measured by sperm chromatin dispersion assay (n = 10 dogs). (d) LH stimulated testosterone secretion by canine testis explants was measured in the presence of each chemical independently. Dotted line (100%) = explant + media only (no LH, no chemical). Chemicals were tested at 2x, 10x and 100x (a–c) or 10x and 100x (d) the concentration detected in testis [mean testis levels for PCB153 0.063 ± 0.01 ng/g (n = 34) & DEHP: 0.182 ± 0.044 ug/g (n = 25)]. (a–d): Predicted values from general ANOVA model are presented. *P < 0.05, **P < 0.01. NS = not significant. Error bar = SED.