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. 2016 Aug 9;6:31204. doi: 10.1038/srep31204

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Copyright © 2016, The Author(s)

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(A) The single-gene-deletion mutants, ΔMocapn7, ΔMocapn9, ΔMocapn14. (B) The double-gene-deletion mutants, ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7. The mutants were verified by double PCR for the targeted gene using the β-tubulin gene as a positive control (~1.0 kb band). The wild-type strain produced a characteristic band, indicating the targeted gene (~0.5 kb band), while the null mutants did not (Upper). The mutants were verified by PCR using a unique recombinational DNA fragment indicating a knockout event. The null mutants have a ~2.0–3.0 kb band on an electrophoretic gel, while the wild-type strain and the ectopic transformants do not (Lower).