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. 2016 Aug 9;6:31294. doi: 10.1038/srep31294

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Copyright © 2016, The Author(s)

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(a) Growth curves and the relative change in the growth for S. cerevisiae containing consensus (WT) and mutatated (S7A) CTD heptad at 30 °C. (b) S7A reduces mRNA transcript capping. The fractional decrease in capped mRNA as well as its relative abundance was measured by doing immunoprecipitation experiment using five micrograms of RNA and H20, an anti-5′ cap monoclonal antibody. The standard deviations are displayed as error bars. (c) Growth curves and the relative change in the growth for S. pombe containing full length consensus (WT) and mutated (S7A) CTD heptad at 30 °C. (d) S7A reduces mRNA transcript capping. The fractional decrease in capped mRNA as well as its relative abundance in S7A as mentioned above.