Table 2. TriTrypDB accessions and annotations for TcEpsinR-associated proteins identified from mass spectrometry.
| Accession | Annotation | Rep 1 | Con1 | Rep 2 | Con 2 | Rep 3 | Con 3 |
|---|---|---|---|---|---|---|---|
| Clathrin | |||||||
| TcCLB.506167.50 | Clathrin HC | 82,17 | 0 | 26,32 | 0,59 | 21,94 | 0,49 |
| TcCLB.506211.240 | Clathrin LC | 1,79 | 0 | 2,61 | 0 | 3,66 | 0,14 |
| TcCLB.510045.30 | Auxilin | 0,27 | 0 | 0 | 0 | 0,17 | 0 |
| Adaptor complex 1 | |||||||
| TcCLB.508257.260 | AP-1γ | 1,71 | 0 | 1,45 | 0,32 | 1,06 | 0,23 |
| TcCLB.506247.200 | AP-1β | 0,88 | 0 | 1,36 | 0 | 0,93 | 0 |
| TcCLB.510533.40 | AP-1μ | 0,85 | 0 | 1,22 | 0 | 1,09 | 0,2 |
| TcCLB.509623.19 | AP-1σ | 0,36 | 0 | 0,59 | 0 | 0,17 | 0 |
| Adaptor complex 4 | |||||||
| TcCLB.511751.200 | AP-4ε | 0,11 | 0 | 0,12 | 0 | 0 | 0 |
| TcCLB.509911.70 | AP-4μ | 0,05 | 0 | 0,24 | 0 | 0,11 | 0 |
| Other adaptors | |||||||
| TcCLB.506925.70 | EpsinR | 12,83 | 0 | 17,08 | 0 | 13,59 | 0 |
| TcCLB.504105.120 | Tepsin | 0,39 | 0 | 1,2 | 0 | 1,2 | 0 |
| TcCLB.503449.30 | AP180/Calm | 0,18 | 0 | 0,12 | 0 | 0 | 0 |
| SNAREs | |||||||
| TcCLB.511627.60 | VAMP7a | 1,26 | 0 | 0,59 | 0 | 0,26 | 0 |
| TcCLB.506855.140 | Vamp7c | 0,74 | 0 | 0,95 | 0 | 0,95 | 0 |
| TcCLB.507811.60 | Vamp7b | 0,45 | 0 | 0,85 | 0 | 0,85 | 0 |
| TcCLB.507795.50 | Syntaxin 7 | 0,22 | 0 | 0,11 | 0 | 0,22 | 0 |
| TcCLB.508955.10 | Qc | 0,22 | 0 | 0,58 | 0 | 0,41 | 0 |
| TcCLB.506401.130 | Qa | 0,16 | 0 | 0,57 | 0 | 0 | 0 |
| TcCLB.508465.120 | Syntaxin 16 | 0,09 | 0 | 0,53 | 0,09 | 0,29 | 0 |
| Rabs | |||||||
| TcCLB.509805.60 | Rab5 | 0 | 0 | 0,64 | 0 | 0,85 | 0 |
| TcCLB.510911.30 | Rab4 | 0,49 | 0 | 0 | 0 | 0,49 | 0 |
| Cargo proteins | |||||||
| TcCLB.511391.180 | GLP-1 | 0,19 | 0 | 2,77 | 0,42 | 3,5 | 0,42 |
| Recycling system | |||||||
| TcCLB.506925.100 | SCAMP domain | 0,15 | 0 | 0 | 0 | 0,15 | 0 |
| Trypanosome specific clathrin-associated proteins | |||||||
| TcCLB.503595.10 | CAP80 | 0,18 | 0 | 0,28 | 0 | 0,13 | 0 |
| TcCLB.507221.70 | CAP141 | 0,18 | 0 | 0,26 | 0 | 0,22 | 0 |
| TcCLB.507895.170 | CAP30 | 0,22 | 0 | 0,22 | 0 | 0,22 | 0 |
| Others | |||||||
| TcCLB.509319.40 | DUF846 | 0,24 | 0 | 0,53 | 0 | 0,53 | 0 |
| TcCLB.503791.49 | Vps45 | 0,21 | 0 | 0,4 | 0 | 0,47 | 0 |
The emPai scores for three independent replicate (Rep) isolations are shown in columns C to H together with concurrent control isolations using cryolysates from untagged cells under the same buffer conditions. Isolation buffer used was 20 mM Hepes 7.4, 250 mM citrate, 0.1% CHAPS, 1 mM MgCl2 10 μM CaCl2, plus protease inhibitor cocktail. Accessions in bold are in common with the clathrin light chain isolation (Table 1). Only proteins identified with a five-fold greater emPai against the control and greater than 0.1 are shown.