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. 2016 Aug 9;6:30839. doi: 10.1038/srep30839

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Copyright © 2016, The Author(s)

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Representative confocal microscopy images of control cells expressing eYFP alone (a) or in combination with the untagged MtSEO-F1 (b) or MtSEO-F4 (c) subunits show cytosolic fluorescence as well as non-fluorescent artificial forisomes (indicated by red asterisks) in (b,c). Yeast cells expressing N-terminal eYFP-MtSEO-F fusion proteins (d–i) or C-terminal MtSEO-F-eYFP fusion proteins (j–o) either alone or in combination with each untagged MtSEO-F protein reveal the most suitable combinations for functionalisation by forming fluorescent forisome structures. (p–s) Yeast cells expressing candidate MtSEO-F combinations as eYFP-glucose-6-phosphate dehydrogenase (G6PDH)-cIL-MtSEO-F fusion proteins for the confirmation of functional forisome assembly when larger enzyme tags are present.