Figure 3. Stimulus-response characteristics and stability of forizymes.

(a) Microscopic images of G6PDH forizyme conformational changes caused by the addition of Ca²⁺ and subsequent removal. (b) Thermal stability of forizymes and soluble G6PDH. Both enzyme preparations were incubated at 40 °C and aliquots were withdrawn at different time points to determine residual enzyme activity. (c) To demonstrate recyclability, forizyme and agarose-immobilised G6PDH activity was monitored during multiple reaction cycles. Forizymes were centrifuged to the bottom of the reaction well prior to the reaction (small picture). Measurements are shown as means ± SD of three independent experiments for each purification. Relative enzyme activity is shown as a percentage of initial activity.