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. 2016 Aug 9;6:30839. doi: 10.1038/srep30839

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Copyright © 2016, The Author(s)

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(a) Vector constructs for the expression of forizymes with two fluorophore-tagged enzymes, and confocal microscopy images of a corresponding yeast cell expressing a Cerulean-HXK2-cIL-MtSEO-F1/eYFP-G6PDH-cIL-MtSEO-F1/MtSEO-F1 forizyme. GPD: gluceraldehyde-3-phosphate dehydrogenase promoter; white arrowhead indicates start codon; black asterisk indicates stop codon; T indicates terminator. (b) Confocal microscopy images of purified forizymes composed of Cerulean-HXK2-cIL-MtSEO-F1/eYFP-G6PDH-cIL-MtSEO-F1/MtSEO-F1. Forizymes contain either both (white in overlay) or only one of the fluorophore-tagged enzyme-MtSEO-F1 fusions (blue or yellow, respectively). (c) Percentage of forizymes composed of Cerulean-HXK2-cIL-MtSEO-F1/MtSEO-F1, eYFP-G6PDH-cIL-MtSEO-F1/MtSEO-F1, or both fluorophore-enzyme fusions. Data are shown as means ± SD of two independent purifications with n = 183 fluorescent forizymes counted.