Figure 3. Cur associated with red united blue light induces reversible cell cycle arrest in A375 cells.

(A) Cell cycle distribution of A375 cells treated with Cur and light irradiation. Cells were treated with Cur alone, Cur combined with red light, Cur combined with blue light and Cur synergized red united blue light irradiation, respectively. The histograms exhibit percent of cells in different phages of cell cycle. (B) Clonogenic survival assay of A375 cells treated with Cur in the absence or presence of light irradiation. A375 cells were treated as described above. 20 h later, the cells were collected and cultured in fresh medium at 1000 cells/well. After 9 days’ culture, cells were fixed with 4% paraformaldehyde and stained with crystal violet. Analysis of colonies per dish, represented as a percent of control. Bars between control groups and treated groups are conspicuously different at p < 0.05(*) or p < 0.01(**) level.