Table 3.
List of primers designed for qRT-PCR validation of RNA-seq data
| Unigene Id | Gene name | Primers |
|---|---|---|
| comp134704_c0_seq1 | Sex determining | F:5ʹAGTCCTGACGCTGGGTATGC 3ʹ |
| Region Y-Box 17 (sox17) | R:5ʹGTCGCCGTATCCGAGGTTC 3ʹ | |
| comp135742_c0_seq2 | SIX homeobox 3a (six3a) | F:5ʹTTATCCCTCCCATTTCTTCCTG 3ʹ |
| R:5ʹGAGAAGTTGAGGGTCGGTAGCT 3ʹ | ||
| comp137301_c0_seq3 | homeobox D10 (HOXD10) | F:5ʹGCTCTCCTGCTGCTAATACCTT 3ʹ |
| R:5ʹCGGCAGTAGTCCACAGGTTT 3ʹ | ||
| comp141980_c0_seq1 | wingless-type MMTV integration site family, member 11B (wnt11b) | F:5ʹGTAGCCTCGGCCACAGCA 3ʹ |
| R:5ʹGCAGACCTCAAGTCCAAATACCT 3ʹ | ||
| comp128400_c0_seq2 | EYA transcriptional coactivator and phosphatase 3 (eya3) | F:5ʹACCCACCTGGCTGCGAGT 3ʹ |
| R:5ʹGGACGAAGCCCTGGAACTG 3ʹ | ||
| comp118626_c0_seq1 | actin, beta (Actb) | F:5ʹAGGTCCTTACGGATGTCAACG 3ʹ |
| R:5ʹGCGTTTCAGGTGCCCAGA 3ʹ |