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Establishing a model of infection of porcine peripheral blood monocytes by H. parasuis. 5 × 105 cells were seeded into the culture plates, then H. parasuis (105, 106, 107 CFU/mL) was added to each well and incubated under 5% CO2 at 37 °C for 3, 6, 12, and 20 h, respectively. Inflammatory cytokines from the supernatant were measured to determine the MOI and optimal interaction time.
