Table 2.

Interactions of CpROMs, TgROM2 and MIC proteins by yeast two-hybrid assay

Bait plasmid	Prey plasmid	Growth on selection plate	β-galactosidase activity
pGBKT7-53	pGADT7-T	+	+
pGBKT7-CpROM1	pGADT7-CpGP900	+	+
pGBKT7-CpROM4	pGADT7-CpGP900	–	–
pGBKT7-CpROM1	pGADT7-CpTRAP-C1	–	–
pGBKT7-CpROM4	pGADT7-CpTRAP-C1	–	–
pGBKT7-TgROM2	pGADT7-CpTRAP-C1	+	+
pGBKT7-Lam	pGADT7-T	–	–

+, growth and/or β-galactosidase activity; –, no growth and/or no β-galactosidase activity