Table 1.

Strains and plasmids used in this study.

Strains	Genotype	References
E. coli BL21 (DE3)	F−, dcm, ompT, hsdS(rB− mB−), gal, (DE3)	Studier and Moffatt, 1986
E. coli BL21 pET15b-His-CobB1	His-CobB1 overexpression strain CmR, AmpR	This work
E. coli BL21 pET15b-His-CobB2	His-CobB2 overexpression strain CmR, AmpR	This work
S. coelicolor M145	S. coelicolor A3(2) spc1− and spc2−	Kieser et al., 2000
S. coelicolor M145 glnR	glnR mutant strain of S. coelicolor M145; glnR replaced by an aac(3)IV cassette, AprR	Tiffert et al., 2011
S. coelicolor M145 pGMStrep-glnR	S. coelicolor M154 with pGM-Strep-glnR, PtipA, KmR	Tiffert et al., 2008
pGM190	Streptomyces- E.coli shuttle vector, TsR, KmR, pSG5 derivative, PtipA	Wohlleben et al., 2009
pET15b	His-(N-term), AmpR, PtipA	Novagen