Table 2.
Results from the “in field” trial: real time amplification mix for different biological fluids were applied on dental mirrors after use, after disinfection in bowl, and after the complete reprocessing procedure
| Used (CT) | Bowl disinfected (CT) | Complete reprocessing (CT) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID | Mix_Es | Mix_S | Mix_V | Hu DNA | ID | Mix_Es | Mix_S | Mix_V | Hu DNA | ID | Mix_Es | Mix_S | Mix_V | Hu DNA |
| 1u | - | +(36.9) | - | +(28.64) | 1d | - | +(39.1) | - | +(27.26) | 23 | - | - | - | NA |
| 2u | - | +(34.3) | - | +(27.27) | 2d | - | +(34.8) | - | +(29.37) | 24 | - | - | - | NA |
| 3u | - | +(37.8) | - | NA | 3d | - | +(37.9) | - | NA | 25 | - | - | - | NA |
| 4u | - | - | - | +(30.4) | 4d | - | +(27.7) | - | NA | 26 | - | - | - | NA |
| 5u | - | - | - | +(29.9) | 5d | - | - | - | NA | 27 | - | - | - | NA |
| 6u | - | +(36.2) | - | NA | 6d | - | - | - | NA | 28 | - | - | - | NA |
| 7u | - | - | - | +(26.8) | 7d | - | - | - | NA | 29 | - | - | - | NA |
| 8u | - | ++(29.1;35.73) | - | +(26.26) | 8d | - | ++(29.1;35.7) | - | +(30.47) | 30 | - | - | - | - |
| 9u | - | ++(28;30.3) | - | +(26.7) | 9d | - | +(36.1) | - | +(27.48) | 31 | - | - | - | - |
| 10u | - | - | - | +(27.2) | 10d | - | - | - | NA | 32 | - | - | - | - |
| 11u | - | +(38.4) | - | +(31.33) | 11d | - | ++(31.3;33.1) | - | NA | 33 | - | - | - | - |
| 12u | - | ++(32.7;37.13) | - | NA | 12d | - | - | - | NA | 34 | - | - | - | - |
| 13u | ++(30.12;23.21) | +(34.7) | - | NA | 13d | - | - | - | NA | 35 | - | - | - | - |
| 14u | +(32.58) | ++(38.29;32.14) | - | +(28.89) | 14d | - | +(34.3) | - | +(34.2) | 36 | - | - | - | NA |
| 15u | +(35.72) | +(33.2) | - | NA | 15d | - | +(34.5) | - | NA | 37 | - | - | - | NA |
| 16u | - | ++(35.7;32.5) | - | +(27.21) | 16d | - | +(33) | - | +(29.19) | 38 | - | - | - | NA |
| 17u | - | +(33.2) | - | NA | 17d | - | ++(35.7;36.8) | - | NA | 39 | - | - | - | NA |
| 18u | - | ++(35.78;36.64) | - | NA | 18d | - | +(35.6) | - | NA | 40 | - | - | - | NA |
| 19u | - | +(34.9) | - | +(27.21) | 19d | +(25.2) | +(36.1) | - | +(27.21) | 41 | - | - | - | NA |
| 20u | - | - | - | - | 20d | - | - | - | NA | 42 | - | - | - | NA |
| 21u | - | +(38.2) | - | NA | 21d | - | - | - | NA | |||||
| 22u | - | +(38.4) | - | NA | 22d | - | - | - | NA | |||||
(−) negative sample; (+) positive sample; (++) positive sample with two indicators of biological fluid (Mix_ES: E. faecalis and S. aureus; Mix_S: S. salivarius and S. mutans), CT values are shown in brackets; NA = Not Applicable; u: used mirror; d: used mirror after immersion in the disinfectant of the temporary storage bowl; HuDNA: Human DNA; additional testing was performed only on borderline doubt samples selected with a CT over 35