Table 1.

Mutation of the Clock^Δ19 gene impacts stool microbiota community structure when mice were fed an alcohol-control diet (i.e., high-fat, high-sugar) or an alcohol-containing diet. Bray-Curtis metric was calculated in a pair-wise fashion and analysis of similarity (ANOSIM) was performed. Global R is based on ANOSIM analysis. P-values are calculated based on a permutations analysis, employing 999 permutations and p –values were false discovery rate corrected for multiple comparisons. WT, wild type mice, Clock^Δ19 mutant mice; Chow, standard rodent chow diet; Control, alcohol-control diet (i.e., high-fat, high-sugar diet); Alcohol, alcohol-containing diet.

		Taxonomic level	Global R	p-value
Standard Chow Diet
WT Chow (n=10)	ClockΔ19 Chow (n=10)	Phylum	0.04	0.24
		Class	0.04	0.24
		Order	0.03	0.26
		Family	0.01	0.28
		Genus	0.01	0.30
Alcohol Control Diet
WT Control (n=8)	ClockΔ19 Control (n=8)	Phylum	0.10	0.13
		Class	0.22	0.03*
		Order	0.22	0.03*
		Family	0.24	0.02*
		Genus	0.21	0.02*
Alcohol Diet
WT Alcohol (n=8)	ClockΔ19 Alcohol (n=8)	Phylum	0.21	0.01*
		Class	0.22	0.01*
		Order	0.22	0.02*
		Family	0.25	0.01*
		Genus	0.30	<0.00*