Fig. 7.

Recognition and killing of C. albicans by alveolar macrophages and neutrophils from cPLA₂α^−/− and cPLA₂α^+/+ mice. The ability of (a) alveolar macrophages and (b) neutrophils from untreated cPLA₂α^+/+ (WT) and cPLA₂α^−/− (KO) mice to recognize and kill GFP-C. albicans in vitro was compared. Levels of clec7a (dectin-1) and clec4n (dectin-2) expression were determined by real-time PCR. (c) Alveolar macrophages were isolated by lavage from WT and KO mice 6 h after challenge with GFP-C. albicans and the % macrophages containing internalized GFP-C. albicans determined by microscopy. Results are mean ± SEM (n = 3–4). *p < 0.05 compared to cells from WT mice