. 2016 Aug 9;17:577. doi: 10.1186/s12864-016-2979-5

Table 1.

Proteins with a significantly lower or higher expression in treatment and control groups

Symbol	Protein description	gi no.	MASCOT score*	Relative intensity^a (normalized)
Symbol	Protein description	gi no.	MASCOT score*	Control	0.0001	0.01	1	100
Energy metabolism (46 %)
ATP5H	ATP synthase subunit d, mitochondrial	gi\|21313679	58	1^A	0.99 ± 0.15^A	0.93 ± 0.12^A	0.63 ± 0.01^A,B	0.36 ± 0.12^B
ATP5O	ATP synthase subunit O, mitochondrial	gi\|20070412	69	1^A	0.97 ± 0.19^A	0.68 ± 0.14^A,B	0.62 ± 0.08^A,B	0.44 ± 0.07^B
HADHA	Trifunctional enzyme subunit alpha, mitochondrial	gi\|33859811	66	1^A	0.71 ± 0.01^A,B	0.41 ± 0.03^B,C	0.40 ± 0.04^B,C	0.31 ± 0.10^C
COX6A1	Cytochrome c oxidase subunit 6A1, mitochondrial	gi\|1352173	49	1^A	0.68 ± 0.19^A,B	0.28 ± 0.07^B,C	0.23 ± 0.06^B,C	0.17 ± 0.01^C
PGAM2	Phosphoglycerate mutase 2	gi\|9256624	53	1^A	0.99 ± 0.13^A	0.76 ± 0.13^A,B	0.72 ± 0.08^A,B	0.34 ± 0.07^B
OXCT2A	Succinyl-CoA:3-ketoacid coenzyme A transferase 2A, mitochondrial	gi\|81881929	36	1^A	0.71 ± 0.16^A,B	0.67 ± 0.07^A,B	0.65 ± 0.04^A,B	0.43 ± 0.09^B
ISOC2A	Isochorismatase domain-containing protein 2A, mitochondrial	gi\|197333728	73	1^A	0.83 ± 0.19^A,B	0.79 ± 0.08^A,B	0.75 ± 0.10^A,B	0.34 ± 0.12^B
GAPDH	Glyceraldehyde-3-phosphate dehydrogenase, testis-specific	gi\|2494630	37	1^A	1.09 ± 0.14^A	1.11 ± 0.25^A,B	1.12 ± 0.31^A,B	2.17 ± 0.28^B
AK2	Adenylate kinase 2, mitochondrial isoform	gi\|34328230	70	1^A	1.13 ± 0.18 ^A,B	1.19 ± 0.27^A,B	1.37 ± 0.11^A,B	2.05 ± 0.14^B
SDHB	Succinate dehydrogenase Ip subunit	gi\|34328286	63	1^A	2.59 ± 0.66^A,B	3.02 ± 0.29^A,B	3.32 ± 0.56^B	3.27 ± 0.38^B
UQCRFS1	Cytochrome b-c1 complex subunit Rieske, mitochondrial	gi\|13385168	62	1^A	1.56 ± 0.17^A,B	1.62 ± 0.40^A,B	1.72 ± 0.32^A,B	2.16 ± 0.01^B
Cytoskeletal/structural proteins (25 %)
ROPN1	Ropporin-1	gi\|74227586	202	1^A	0.88 ± 0.12^A	0.78 ± 0.10^A,B	0.71 ± 0.10^A,B	0.49 ± 0.01^B
ACTB	Actin, cytoplasmic 1	gi\|6671509	51	1^A	0.93 ± 0.17^A	0.92 ± 0.16^A	0.49 ± 0.04^B	0.47 ± 0.04^B
FABP9	Fatty acid-binding protein 9	gi\|166897974	68	1^A	0.94 ± 0.05^A	0.86 ± 0.13^A	0.68 ± 0.03^A,B	0.47 ± 0.07^B
ODF2	Outer dense fiber protein 2	gi\|2290719	57	1^A	0.68 ± 0.14^A,B	0.62 ± 0.19^A,B	0.59 ± 0.16^A,B	0.13 ± 0.01^B
PMP20	Peroxisomal membrane protein 20	gi\|6746357	79	1^A	1.06 ± 0.05^A	1.10 ± 0.06^A,B	1.17 ± 0.22^A,B	2.11 ± 0.01^B
ASRGL1	Isoaspartyl peptidase/L-asparaginase	gi\|81875980	52	1^A	1.38 ± 0.12^A,B	2.09 ± 0.87^A,B	2.79 ± 0.42^A,B	3.23 ± 0.36^B
Fertility Related Proteins (17 %)
PHB	Prohibitin	gi\|6679299	68	1^A	0.91 ± 0.12^A	0.81 ± 0.20^A,B	0.75 ± 0.12^A,B	0.43 ± 0.05^B
PPP1CA	Serine/threonine-protein phosphatase PP1-alpha catalytic subunit	gi\|49065812	50	1^A	0.94 ± 0.16^A	0.60 ± 0.20^A,B	0.47 ± 0.07^A,B	0.36 ± 0.05^B
DNAL1	Dynein light chain 1, axonemal	gi\|164607162	67	1^A	0.74 ± 0.01^A,B	0.65 ± 0.14 ^A,B	0.64 ± 0.23^A,B	0.49 ± 0.13^B
SPA17	Sperm surface protein Sp17	gi\|6755614	58	1^A	1.11 ± 0.21^A	1.39 ± 0.81^A	1.59 ± 0.05^A,B	2.08 ± 0.17^B
Stress response proteins/ROS metabolism (12 %)
GSTM5	Glutathione S-transferase Mu 5	gi\|6754086	70	1^A	0.92 ± 0.26^A,B	0.67 ± 0.08^A,B	0.48 ± 0.09^B	0.32 ± 0.08^B
GPX4	Glutathione peroxidase	gi\|3075477	65	1^A	1.71 ± 0.08^A,B	1.87 ± 0.19^B	2.24 ± 0.24^B	2.25 ± 0.15^B
PRDX5	Peroxiredoxin-5, mitochondrial	gi\|6755114	73	1^A	1.28 ± 0.17^A,B	1.30 ± 0.15^A,B	2.02 ± 0.11^B	2.35 ± 0.09^B

*MASCOT score is −10 log (P), where P is the probability that the observed match is a random event. Individual scores > 30 indicate identity or extensive homology (p < 0.05). ^aRelative spots intensity in control and BPA-treated (μM) spermatozoa. Data are presented as mean ± SEM (3 replicates). Values with different superscript characters (A,B,C) indicate significant differences between the control and treatment groups as determined by one-way ANOVA (P < 0.05)