Figure 1.
Severe AKI leads to poor outcome. Three-month-old WT mice underwent Bi-IRI with different duration of ischemia and then were fed normal rodent chow for ≤28 days. (A) Survival rate. (B) ClCr. Data are expressed as means±SDs of at least four mice from each group, and statistical significance was assessed by one-way ANOVA followed by Newman–Keuls test, and accepted when *P<0.05 versus 20 minutes; #P<0.05 versus 25 minutes on the same day. BW, body weight. (C) Representative Trichrome stain of kidney sections at 14 days post-AKI. (D) Renal fibrosis scores obtained from a Trichrome-stained section with ImageJ. Data are expressed as means±SDs of four mice from each group, and statistical significance was assessed by one-way ANOVA followed by Newman–Keuls test, and accepted when **P<0.01 between two groups.