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. 2015 Dec 23;27(8):2331–2345. doi: 10.1681/ASN.2015060613

Figure 4.

Figure 4.

Low renal αKlotho is associated with more severe renal fibrosis in mice fed with a high Pi diet. Three-month-old WT mice underwent Bi-IRI, Npx-IRI, or laparotomy for sham and were fed normal–phosphate rodent chow (NP; 0.7%) or high-Pi diet (HP; 2.0% Pi) for 18 weeks starting 2 weeks after surgery. (A) PPi. (B) ClCr. BW, body weight. (C) Renal fibrosis at 20 weeks. (Upper panel) Representative micrographs of Trichrome staining in the kidney sections. Scale bar, 500 μm. (Lower panel) Renal fibrosis scores were obtained from Trichrome-stained sections with ImageJ. (D) αKlotho and fibrotic markers in the kidney. (Left panel) Representative immunoblotting for αKlotho, α-SMA, CTGF, Col I, and β-actin protein. (Right panel) Summary of immunoblots in arbitrary units from all immunoblots. Data are expressed as means±SDs of at least four mice from each group, and statistical significance was assessed by one-way ANOVA followed by Newman–Keuls test. Significant difference was accepted when *P<0.05; **P<0.01 between two groups.