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. 2015 Dec 23;27(8):2331–2345. doi: 10.1681/ASN.2015060613

Figure 6.

Figure 6.

Soluble αKlotho protein promotes renal recovery and mitigates renal fibrosis in WT mice after Bi-IRI. (A) Three-month-old WT mice were intraperitoneally given αKlotho protein (0.01 mg/kg) for 4 consecutive days starting 24 hours after Bi-IRI. Mice were euthanized at 14, 28, and 140 days after surgery. (B) ClCr. BW, body weight. Data are expressed as means±SDs of at least four mice from each group, and statistical significance was assessed by unpaired t test. Statistical significance was accepted when *P<0.05; **P<0.01 versus vehicle. (C) Renal fibrosis at 14 and 28 days. (Upper panel) Representative micrographs of Trichrome staining in the kidney sections. Scale bar, 500 μm. (Lower panel) Renal fibrosis scores were obtained from Trichrome-stained sections with ImageJ. (D) αKlotho and fibrotic markers in the kidney. (Left panel) Representative immunoblotting for αKlotho, α-SMA, CTGF, Col I, and β-actin protein. (Right panel) Summary of arbitrary units from all immunoblots. Data are expressed as means±SDs of four mice from each group, and statistical significance was assessed by one-way ANOVA followed by Newman–Keuls test. Statistical significance was accepted when *P<0.05; **P<0.01 between two groups.