Figure 4.

Role of TNF-α generated by the renal epithelium in the development of cisplatin–induced renal damage. (A and B) Kidney function in cisplatin–treated WT and TNF KKO (Ksp TNF KO) mice as measured by (A) BUN and (B) sCr. (C–E) Renal pathology in cisplatin-treated animals. Representative images of kidney sections from cisplatin–treated (C) WT and (D) TNF KKO mice with (E) a summary of renal injury scores (n=3 for saline and n≥17 for cisplatin). (F and G) Renal mRNA expression of (F) NGAL and (G) TNF-α (n=3 for saline and n≥9 for cisplatin). (H) Serum and (I) kidney TNF-α protein content (n=3 for saline and n=8 for cisplatin). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.