FIGURE 9.

Loss of Bcl-2 enhances β-cell glucose responses. (A) Quantitative PCR quantification of Bcl-2 and Bcl-xL mRNA levels in islets from Bcl-2^+/- and Bcl-2^−/− islets compared to Bcl-2^+/+ islets. (B) Average cytosolic Ca2+ levels of dispersed islet cells from Bcl-2^+/+, Bcl-2^+/−, and Bcl-2^−/− islets. (C) Incremental area under the curve of Ca²⁺ responses by Bcl-2^+/+, Bcl-2^+/−, and Bcl-2^−/− islets. (D) Integrated cytosolic Ca2+ responses of Bcl-2^−/− and Bcl-2^+/+ β-cells depolarized with 30 mM KCl, (E and F) integrated Ca²⁺ and NAD(P)H autofluorescence increases of intact islet cells, normalized Bcl-2^+/+ control islet cells. (G) Insulin secretion profiles of perifused islets from Bcl-2^+/+ and Bcl-2^−/− islets. (H) Quantified area under the curve of insulin secretion profiles by Bcl-2+/+ and Bcl-2^−/− islets. From Luciani DS, et al. Diabetes 2013;62:170-82.